Review



immunomagnetic bead based sorting  (Miltenyi Biotec)


Bioz Verified Symbol Miltenyi Biotec is a verified supplier
Bioz Manufacturer Symbol Miltenyi Biotec manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    Miltenyi Biotec immunomagnetic bead based sorting
    Immunomagnetic Bead Based Sorting, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 915 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+beads/CD19+MicroBeads%2C+mouse/pm41966487-72-9-12
    Average 96 stars, based on 915 article reviews
    immunomagnetic bead based sorting - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Red Blood Cell Lysis:

    Article Title: A post-translational cysteine-to-serine conversion in human and mouse insulin generates a diabetogenic neoepitope
    Article Snippet: .. After red blood cell lysis, cells were washed with MACS buffer, and the CD19 + B cells and CD11b + myeloid cells were depleted using CD19 beads (Miltenyi Biotec, Cat #: 130-050-301) and CD11b beads (Miltenyi Biotec, Cat#: 130-097-142). ..

    Article Title: A microenvironment-driven HLA-II-associated insulin neoantigen elicits persistent memory T cell activation in diabetes
    Article Snippet: .. After red blood cell lysis, cells were washed with MACS buffer and CD19 + B cells and CD11b + myeloid cells were depleted using CD19 beads (Miltenyi Biotec, Cat130-050-301) and CD11b beads (Miltenyi Biotec, Cat#130-097-142). ..

    Article Title: A microenvironment-driven HLA-II-associated insulin neoantigen elicits persistent memory T cell activation in diabetes.
    Article Snippet: .. After red blood cell lysis, cells were washed with MACS buffer and CD19+ B cells and CD11b+ myeloid cells were depleted using CD19 beads (Miltenyi Biotec, Cat130-050-301) and CD11b beads (Miltenyi Biotec, Cat#130-097-142). ..

    Article Title: Crinophagic granules in pancreatic β cells contribute to mouse autoimmune diabetes by diversifying pathogenic epitope repertoire
    Article Snippet: .. After red blood cell lysis, cells were washed with MACS buffer, and the CD19 + B cells and CD11b + myeloid cells were depleted by CD19 beads (Miltenyi Biotec, Cat #: 130-050-301, 120 μl for each sample) and CD11b beads (Miltenyi Biotec, Cat #: 130-097-142, 80 μl for each sample). ..

    Magnetic Cell Separation:

    Article Title: A post-translational cysteine-to-serine conversion in human and mouse insulin generates a diabetogenic neoepitope
    Article Snippet: .. After red blood cell lysis, cells were washed with MACS buffer, and the CD19 + B cells and CD11b + myeloid cells were depleted using CD19 beads (Miltenyi Biotec, Cat #: 130-050-301) and CD11b beads (Miltenyi Biotec, Cat#: 130-097-142). ..

    Article Title: A microenvironment-driven HLA-II-associated insulin neoantigen elicits persistent memory T cell activation in diabetes
    Article Snippet: .. After red blood cell lysis, cells were washed with MACS buffer and CD19 + B cells and CD11b + myeloid cells were depleted using CD19 beads (Miltenyi Biotec, Cat130-050-301) and CD11b beads (Miltenyi Biotec, Cat#130-097-142). ..

    Article Title: A microenvironment-driven HLA-II-associated insulin neoantigen elicits persistent memory T cell activation in diabetes.
    Article Snippet: .. After red blood cell lysis, cells were washed with MACS buffer and CD19+ B cells and CD11b+ myeloid cells were depleted using CD19 beads (Miltenyi Biotec, Cat130-050-301) and CD11b beads (Miltenyi Biotec, Cat#130-097-142). ..

    Article Title: Crinophagic granules in pancreatic β cells contribute to mouse autoimmune diabetes by diversifying pathogenic epitope repertoire
    Article Snippet: .. After red blood cell lysis, cells were washed with MACS buffer, and the CD19 + B cells and CD11b + myeloid cells were depleted by CD19 beads (Miltenyi Biotec, Cat #: 130-050-301, 120 μl for each sample) and CD11b beads (Miltenyi Biotec, Cat #: 130-097-142, 80 μl for each sample). ..

    Transformation Assay:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Generated:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15. .. NALM6 was transduced with the pCDH-EF1α-Luciferase-T2A-GFP lentiviral vector and sorted using a BD FACSAria (Becton Dickinson).

    FACS:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15. .. NALM6 was transduced with the pCDH-EF1α-Luciferase-T2A-GFP lentiviral vector and sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
    Article Snippet: Induction of FCRL4+ B cells in vitro Human peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation with ficoll paque plus (Cytiva,17-1440-02). .. Total B cells were isolated by magnetic activated cell sorting with CD19 Beads (Miltenyi Biotec, 130-050- 301) and cultured in 96-well round bottom plates. .. B cells were left untreated or treated with 5ng/mL LPS (Sigma-Aldrich, L4391), 5ng/mL poly I:C (Sigma-Adrich, P0913), 50% culture supernatant from SW480 or Huh7 cell line, 5ng/mL CpG (Invivogen, tlrl2006), 0.3ug/mL CD40 antibody (R&D, AF632-SP), 0.01 mM CEACAM5 (CEA) Protein (SINO BIOLOGICAL,11077-H08H), the combination of CpG and anti-CD40, or the combination of CpG, anti-CD40 and CEACAM5.

    Infection:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15. .. NALM6 was transduced with the pCDH-EF1α-Luciferase-T2A-GFP lentiviral vector and sorted using a BD FACSAria (Becton Dickinson).

    Virus:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Produced:

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV transformed lymphoblastoid 438 cell lines (LCL) from allogeneic human fetal liver tissue were generated by isolating CD19+ cells by 439 magnetic activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with 440 Epstein-Barr Virus (EBV) B95-8; produced as previously described57 at a multiplicity of infection (MOI) 441 of 0.05-0.15. .. NALM6 was transduced with the pCDH-EF1-Luciferase-T2A-GFP lentiviral vector and 442 sorted using a BD FACSAria (Becton Dickinson).

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells
    Article Snippet: .. EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15. .. NALM6 was transduced with the pCDH-EF1α-Luciferase-T2A-GFP lentiviral vector and sorted using a BD FACSAria (Becton Dickinson).

    Isolation:

    Article Title: PGRN Inhibits Early B‐cell Activation and IgE Production Through the IFITM3‐STAT1 Signaling Pathway in Asthma
    Article Snippet: .. In this experiment, human peripheral blood mononuclear cells (PBMCs) were isolated and CD19 beads (130‐050‐301; Miltenyi) were used to select B cells. ..

    Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
    Article Snippet: Induction of FCRL4+ B cells in vitro Human peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation with ficoll paque plus (Cytiva,17-1440-02). .. Total B cells were isolated by magnetic activated cell sorting with CD19 Beads (Miltenyi Biotec, 130-050- 301) and cultured in 96-well round bottom plates. .. B cells were left untreated or treated with 5ng/mL LPS (Sigma-Aldrich, L4391), 5ng/mL poly I:C (Sigma-Adrich, P0913), 50% culture supernatant from SW480 or Huh7 cell line, 5ng/mL CpG (Invivogen, tlrl2006), 0.3ug/mL CD40 antibody (R&D, AF632-SP), 0.01 mM CEACAM5 (CEA) Protein (SINO BIOLOGICAL,11077-H08H), the combination of CpG and anti-CD40, or the combination of CpG, anti-CD40 and CEACAM5.

    Cell Culture:

    Article Title: Pan-cancer single-cell dissection reveals phenotypically distinct B cell subtypes.
    Article Snippet: Induction of FCRL4+ B cells in vitro Human peripheral blood mononuclear cells (PBMCs) were isolated by density gradient centrifugation with ficoll paque plus (Cytiva,17-1440-02). .. Total B cells were isolated by magnetic activated cell sorting with CD19 Beads (Miltenyi Biotec, 130-050- 301) and cultured in 96-well round bottom plates. .. B cells were left untreated or treated with 5ng/mL LPS (Sigma-Aldrich, L4391), 5ng/mL poly I:C (Sigma-Adrich, P0913), 50% culture supernatant from SW480 or Huh7 cell line, 5ng/mL CpG (Invivogen, tlrl2006), 0.3ug/mL CD40 antibody (R&D, AF632-SP), 0.01 mM CEACAM5 (CEA) Protein (SINO BIOLOGICAL,11077-H08H), the combination of CpG and anti-CD40, or the combination of CpG, anti-CD40 and CEACAM5.



    Similar Products

    96
    Miltenyi Biotec immunomagnetic bead based sorting
    Immunomagnetic Bead Based Sorting, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+beads/CD19+MicroBeads%2C+mouse/pm41966487-72-9-12
    Average 96 stars, based on 1 article reviews
    immunomagnetic bead based sorting - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec cd19 beads
    NKG2A-edited CAR NK cells eliminate <t>CD19-negative</t> target cells resistant to conventional CAR NK cells (A) Specific lysis of 721.45 CD19 KO cells by engineered NK cells (left) or CAR NK cells (right). Cells were co-cultured for 4–5 h at an effector to target ratio of 5:1. (B) Schematic of repetitive stimulation assay. 0.1 × 10 6 NK or CAR NK cells were seeded at day 0, and 0.1 × 10 6 <t>CD19-positive</t> 721.45 cells (target cells) were added every 2–3 days for 14 days. IL-2 was added 2×/week until day 12. Target cells added on day 7 were labeled with PKH26 and on day 14 with PKH67. Data shown in (C–H) were generated using this setup. (C) Expression of CD19 (histograms) and relative MFI of CD19 on total target cells (live CD56 − ) and representative flow cytometry plots on day 14 after co-culture with NK (top) or CAR NK (bottom) cells. Relative MFI is calculated by dividing by MFI of CD19 on target cells cultured alone. (D) Representative expression of PKH26 (target cells added day 7) and PKH67 (target cells added day 14) on total target cells (live CD56 − ) after co-culture with NK or CAR NK cells for 14 days. (E and F) Frequency of remaining live PKH26 + target cells (added on day 7) on day 14 after co-culture with NK cells (E) or CAR NK cells (F). Target cells alone (721.45 only) were not included in the statistical analysis. (G and H) Frequency of remaining live PKH67 + target cells (added on day 14 and co-cultured for 4–5 h before readout) after co-culture with NK cells (G) or CAR NK cells (H). Target cells alone (721.45 only) were not included in the statistical analysis. (A–H) n = 6–9 (individual donors). One-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    Cd19 Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+beads/CD19+MicroBeads%2C+human/pmc12859412-247-20-22
    Average 96 stars, based on 1 article reviews
    cd19 beads - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec magnetic beads
    NKG2A-edited CAR NK cells eliminate <t>CD19-negative</t> target cells resistant to conventional CAR NK cells (A) Specific lysis of 721.45 CD19 KO cells by engineered NK cells (left) or CAR NK cells (right). Cells were co-cultured for 4–5 h at an effector to target ratio of 5:1. (B) Schematic of repetitive stimulation assay. 0.1 × 10 6 NK or CAR NK cells were seeded at day 0, and 0.1 × 10 6 <t>CD19-positive</t> 721.45 cells (target cells) were added every 2–3 days for 14 days. IL-2 was added 2×/week until day 12. Target cells added on day 7 were labeled with PKH26 and on day 14 with PKH67. Data shown in (C–H) were generated using this setup. (C) Expression of CD19 (histograms) and relative MFI of CD19 on total target cells (live CD56 − ) and representative flow cytometry plots on day 14 after co-culture with NK (top) or CAR NK (bottom) cells. Relative MFI is calculated by dividing by MFI of CD19 on target cells cultured alone. (D) Representative expression of PKH26 (target cells added day 7) and PKH67 (target cells added day 14) on total target cells (live CD56 − ) after co-culture with NK or CAR NK cells for 14 days. (E and F) Frequency of remaining live PKH26 + target cells (added on day 7) on day 14 after co-culture with NK cells (E) or CAR NK cells (F). Target cells alone (721.45 only) were not included in the statistical analysis. (G and H) Frequency of remaining live PKH67 + target cells (added on day 14 and co-cultured for 4–5 h before readout) after co-culture with NK cells (G) or CAR NK cells (H). Target cells alone (721.45 only) were not included in the statistical analysis. (A–H) n = 6–9 (individual donors). One-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+beads/CD19+MicroBeads%2C+human/pmc12938334-130-18-20
    Average 96 stars, based on 1 article reviews
    magnetic beads - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec magnetic beads conjugated to cd19 antibodies
    NKG2A-edited CAR NK cells eliminate <t>CD19-negative</t> target cells resistant to conventional CAR NK cells (A) Specific lysis of 721.45 CD19 KO cells by engineered NK cells (left) or CAR NK cells (right). Cells were co-cultured for 4–5 h at an effector to target ratio of 5:1. (B) Schematic of repetitive stimulation assay. 0.1 × 10 6 NK or CAR NK cells were seeded at day 0, and 0.1 × 10 6 <t>CD19-positive</t> 721.45 cells (target cells) were added every 2–3 days for 14 days. IL-2 was added 2×/week until day 12. Target cells added on day 7 were labeled with PKH26 and on day 14 with PKH67. Data shown in (C–H) were generated using this setup. (C) Expression of CD19 (histograms) and relative MFI of CD19 on total target cells (live CD56 − ) and representative flow cytometry plots on day 14 after co-culture with NK (top) or CAR NK (bottom) cells. Relative MFI is calculated by dividing by MFI of CD19 on target cells cultured alone. (D) Representative expression of PKH26 (target cells added day 7) and PKH67 (target cells added day 14) on total target cells (live CD56 − ) after co-culture with NK or CAR NK cells for 14 days. (E and F) Frequency of remaining live PKH26 + target cells (added on day 7) on day 14 after co-culture with NK cells (E) or CAR NK cells (F). Target cells alone (721.45 only) were not included in the statistical analysis. (G and H) Frequency of remaining live PKH67 + target cells (added on day 14 and co-cultured for 4–5 h before readout) after co-culture with NK cells (G) or CAR NK cells (H). Target cells alone (721.45 only) were not included in the statistical analysis. (A–H) n = 6–9 (individual donors). One-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    Magnetic Beads Conjugated To Cd19 Antibodies, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+beads/B+Cell+Isolation+Kit+II%2C+human/pm41547950-363-13-19
    Average 96 stars, based on 1 article reviews
    magnetic beads conjugated to cd19 antibodies - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec mouse cd19 micro beads
    NKG2A-edited CAR NK cells eliminate <t>CD19-negative</t> target cells resistant to conventional CAR NK cells (A) Specific lysis of 721.45 CD19 KO cells by engineered NK cells (left) or CAR NK cells (right). Cells were co-cultured for 4–5 h at an effector to target ratio of 5:1. (B) Schematic of repetitive stimulation assay. 0.1 × 10 6 NK or CAR NK cells were seeded at day 0, and 0.1 × 10 6 <t>CD19-positive</t> 721.45 cells (target cells) were added every 2–3 days for 14 days. IL-2 was added 2×/week until day 12. Target cells added on day 7 were labeled with PKH26 and on day 14 with PKH67. Data shown in (C–H) were generated using this setup. (C) Expression of CD19 (histograms) and relative MFI of CD19 on total target cells (live CD56 − ) and representative flow cytometry plots on day 14 after co-culture with NK (top) or CAR NK (bottom) cells. Relative MFI is calculated by dividing by MFI of CD19 on target cells cultured alone. (D) Representative expression of PKH26 (target cells added day 7) and PKH67 (target cells added day 14) on total target cells (live CD56 − ) after co-culture with NK or CAR NK cells for 14 days. (E and F) Frequency of remaining live PKH26 + target cells (added on day 7) on day 14 after co-culture with NK cells (E) or CAR NK cells (F). Target cells alone (721.45 only) were not included in the statistical analysis. (G and H) Frequency of remaining live PKH67 + target cells (added on day 14 and co-cultured for 4–5 h before readout) after co-culture with NK cells (G) or CAR NK cells (H). Target cells alone (721.45 only) were not included in the statistical analysis. (A–H) n = 6–9 (individual donors). One-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    Mouse Cd19 Micro Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+beads/CD19+MicroBeads%2C+mouse/bio_rxiv__64898__2025__12__29__696857-185-11-14
    Average 96 stars, based on 1 article reviews
    mouse cd19 micro beads - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    96
    Miltenyi Biotec cd19 magnetic beads
    NKG2A-edited CAR NK cells eliminate <t>CD19-negative</t> target cells resistant to conventional CAR NK cells (A) Specific lysis of 721.45 CD19 KO cells by engineered NK cells (left) or CAR NK cells (right). Cells were co-cultured for 4–5 h at an effector to target ratio of 5:1. (B) Schematic of repetitive stimulation assay. 0.1 × 10 6 NK or CAR NK cells were seeded at day 0, and 0.1 × 10 6 <t>CD19-positive</t> 721.45 cells (target cells) were added every 2–3 days for 14 days. IL-2 was added 2×/week until day 12. Target cells added on day 7 were labeled with PKH26 and on day 14 with PKH67. Data shown in (C–H) were generated using this setup. (C) Expression of CD19 (histograms) and relative MFI of CD19 on total target cells (live CD56 − ) and representative flow cytometry plots on day 14 after co-culture with NK (top) or CAR NK (bottom) cells. Relative MFI is calculated by dividing by MFI of CD19 on target cells cultured alone. (D) Representative expression of PKH26 (target cells added day 7) and PKH67 (target cells added day 14) on total target cells (live CD56 − ) after co-culture with NK or CAR NK cells for 14 days. (E and F) Frequency of remaining live PKH26 + target cells (added on day 7) on day 14 after co-culture with NK cells (E) or CAR NK cells (F). Target cells alone (721.45 only) were not included in the statistical analysis. (G and H) Frequency of remaining live PKH67 + target cells (added on day 14 and co-cultured for 4–5 h before readout) after co-culture with NK cells (G) or CAR NK cells (H). Target cells alone (721.45 only) were not included in the statistical analysis. (A–H) n = 6–9 (individual donors). One-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.
    Cd19 Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd19+beads/CD19+MicroBeads%2C+human/pm41417117-146-12-15
    Average 96 stars, based on 1 article reviews
    cd19 magnetic beads - by Bioz Stars, 2026-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    NKG2A-edited CAR NK cells eliminate CD19-negative target cells resistant to conventional CAR NK cells (A) Specific lysis of 721.45 CD19 KO cells by engineered NK cells (left) or CAR NK cells (right). Cells were co-cultured for 4–5 h at an effector to target ratio of 5:1. (B) Schematic of repetitive stimulation assay. 0.1 × 10 6 NK or CAR NK cells were seeded at day 0, and 0.1 × 10 6 CD19-positive 721.45 cells (target cells) were added every 2–3 days for 14 days. IL-2 was added 2×/week until day 12. Target cells added on day 7 were labeled with PKH26 and on day 14 with PKH67. Data shown in (C–H) were generated using this setup. (C) Expression of CD19 (histograms) and relative MFI of CD19 on total target cells (live CD56 − ) and representative flow cytometry plots on day 14 after co-culture with NK (top) or CAR NK (bottom) cells. Relative MFI is calculated by dividing by MFI of CD19 on target cells cultured alone. (D) Representative expression of PKH26 (target cells added day 7) and PKH67 (target cells added day 14) on total target cells (live CD56 − ) after co-culture with NK or CAR NK cells for 14 days. (E and F) Frequency of remaining live PKH26 + target cells (added on day 7) on day 14 after co-culture with NK cells (E) or CAR NK cells (F). Target cells alone (721.45 only) were not included in the statistical analysis. (G and H) Frequency of remaining live PKH67 + target cells (added on day 14 and co-cultured for 4–5 h before readout) after co-culture with NK cells (G) or CAR NK cells (H). Target cells alone (721.45 only) were not included in the statistical analysis. (A–H) n = 6–9 (individual donors). One-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Journal: Molecular Therapy Oncology

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells

    doi: 10.1016/j.omton.2026.201126

    Figure Lengend Snippet: NKG2A-edited CAR NK cells eliminate CD19-negative target cells resistant to conventional CAR NK cells (A) Specific lysis of 721.45 CD19 KO cells by engineered NK cells (left) or CAR NK cells (right). Cells were co-cultured for 4–5 h at an effector to target ratio of 5:1. (B) Schematic of repetitive stimulation assay. 0.1 × 10 6 NK or CAR NK cells were seeded at day 0, and 0.1 × 10 6 CD19-positive 721.45 cells (target cells) were added every 2–3 days for 14 days. IL-2 was added 2×/week until day 12. Target cells added on day 7 were labeled with PKH26 and on day 14 with PKH67. Data shown in (C–H) were generated using this setup. (C) Expression of CD19 (histograms) and relative MFI of CD19 on total target cells (live CD56 − ) and representative flow cytometry plots on day 14 after co-culture with NK (top) or CAR NK (bottom) cells. Relative MFI is calculated by dividing by MFI of CD19 on target cells cultured alone. (D) Representative expression of PKH26 (target cells added day 7) and PKH67 (target cells added day 14) on total target cells (live CD56 − ) after co-culture with NK or CAR NK cells for 14 days. (E and F) Frequency of remaining live PKH26 + target cells (added on day 7) on day 14 after co-culture with NK cells (E) or CAR NK cells (F). Target cells alone (721.45 only) were not included in the statistical analysis. (G and H) Frequency of remaining live PKH67 + target cells (added on day 14 and co-cultured for 4–5 h before readout) after co-culture with NK cells (G) or CAR NK cells (H). Target cells alone (721.45 only) were not included in the statistical analysis. (A–H) n = 6–9 (individual donors). One-way ANOVA. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001.

    Article Snippet: EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15.

    Techniques: Lysis, Cell Culture, Labeling, Generated, Expressing, Flow Cytometry, Co-Culture Assay

    Increased cytotoxicity of NKG2A-edited CAR NK cells against CD19-negative BCP-ALL PDX (A) CD19 and (B) HLA-E expression of BCP-ALL PDX by flow cytometry. (C) Specific lysis of PDX1 and PDX2 after co-culture with control NK cells (NK mock) or CAR NK cells (CAR mock). Co-culture at E:T ratio 5:1 for 4 h. (D) Specific lysis of PDX1 after 4 h co-culture at 1:1 E:T ratio. (E) Data from (D) shown as relative increase in killing of engineered CAR NK cells compared with controls (CAR mock). (F–H) Specific lysis of PDX2 after 20 h co-culture at 5:1 E:T ratio with NK (F) or CAR NK (G) cells. (H) Data from (G) shown as relative increase in killing of engineered CAR NK cells compared with controls (CAR mock). (C) n = 3 (individual donors), paired t test. (D–G) n = 3 (individual donors), repeated measures one-way ANOVA. Engineered NK cells shown in this figure were generated from bulk NK cells (not NKG2A + NK cells) as the starting population. ∗ p < 0.05.

    Journal: Molecular Therapy Oncology

    Article Title: Co-editing of NKG2A and FAS increases long-term cytotoxic capacity and persistence of CAR NK cells

    doi: 10.1016/j.omton.2026.201126

    Figure Lengend Snippet: Increased cytotoxicity of NKG2A-edited CAR NK cells against CD19-negative BCP-ALL PDX (A) CD19 and (B) HLA-E expression of BCP-ALL PDX by flow cytometry. (C) Specific lysis of PDX1 and PDX2 after co-culture with control NK cells (NK mock) or CAR NK cells (CAR mock). Co-culture at E:T ratio 5:1 for 4 h. (D) Specific lysis of PDX1 after 4 h co-culture at 1:1 E:T ratio. (E) Data from (D) shown as relative increase in killing of engineered CAR NK cells compared with controls (CAR mock). (F–H) Specific lysis of PDX2 after 20 h co-culture at 5:1 E:T ratio with NK (F) or CAR NK (G) cells. (H) Data from (G) shown as relative increase in killing of engineered CAR NK cells compared with controls (CAR mock). (C) n = 3 (individual donors), paired t test. (D–G) n = 3 (individual donors), repeated measures one-way ANOVA. Engineered NK cells shown in this figure were generated from bulk NK cells (not NKG2A + NK cells) as the starting population. ∗ p < 0.05.

    Article Snippet: EBV-transformed LCLs from allogeneic human fetal liver tissue were generated by isolating CD19 + cells by magnetic-activated cell sorting with CD19 beads (Miltenyi, Cat. 130-050-301) and then infected with EBV B95-8, produced as previously described at a multiplicity of infection (MOI) of 0.05–0.15.

    Techniques: Expressing, Flow Cytometry, Lysis, Co-Culture Assay, Control, Generated